my part of sharing diagnostic

My part of sharing: (Diagnostic Bactriology)

Urine Culture:

Urine sample is collected when the patient( in/out patient) is

suspected of urinary tract infection and asymptomatic patients with

high risk of infection.

Common Aetiologic agents: E.Coli, Enterococcus Spp, Klebsiella Spp,

Enterobacter Spp, proteus Spp and Pseudomonas Spp.

Urine Specimen: Midstream urine (2-15ml), collected in sterile plastic

wide-mouthed container. The use of urine bags are discouraged as it

has a high chance of leakage and it will become messy when the

specimen needs to be used in the lab. The specimen must reach the lab

within 2hours/ must be refrigerator upto 24hours. All the relevant

clinical information about the specimen must be provided, recorded and

documented. When plating of urine specimen for viable count we use 2

media (MacConkey and CLED agar). The MacConkey agar acts as the

general media and CLED agar is used as the selective media.

Quantitation is very important for urine culture as it will tells us

if we need to take result into consideration or do not report as

sometimes there might be too many colonies because of worng collection

process. (eg: the specimen is not mid-stream urine). to ensure

quantitation is standardised throughout, we use the same volume of

inoculation tube.(eg: 1 or 10 ul)

Microscopy: 1/> organism/ high power field (hpf) [corresponds to > or

= to 100,000 organism/ml]

Specimen Rejection: the specimen is also rejected if the following

occurs:

1. Important data on the specimen container and request form do not

match.

2. Specimen is unsuitable for test requested

3. Gross spillage of specimen

4. Insufficient specimen available

5. If the agar is soaked in urine (dip stick)

Things to take note:

1.Pyuria, viable counts on the culture, symptoms and patient's factors

(age, presence of catheter, underlying structural abnormalities and

immunologic status) all these must be considered before interpreting

the results.

2. CLED and MacConkey agar may be used if delay in transport is

antisipated.

3. Always streak on the general media before using the same inoculatin

tube to streak on the selective media. This is because selective media

contains inhibitory substances which might also come into contact with

general media. Thus it defeats the whole purpose of culturing in 2

different media.

Done by: Devi :)

posted by Guinea Piggy~ @ 8:31 PM

5 Comments:

* At 2/7/06 5:41 PM, Blogger Guinea Piggy~ said...

To: Devi

What does pyuria means?

Siang Fang